Related Experiment Video
Updated: Jan 11, 2026
Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
Published on: August 15, 2025
A Low-Molecular-Weight Polysaccharide From Cremastra Appendiculata: Extraction, Structural Characterization, and
Haoyue Kong1, Wenna Li1, Yufei Wang1
1National Engineering Laboratory For Resource Development of Endangered Crude, Drugs in Northwest China; Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry; College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Cremastra appendiculata polysaccharide (CAP) shows promise in fighting liver cancer. This natural compound inhibits tumor growth and enhances immune function, offering a potential new treatment for hepatocellular carcinoma (HCC).
Area of Science:
- Pharmacology
- Immunology
- Natural Products Chemistry
Background:
- Cremastra appendiculata is traditionally used for various cancers.
- The antitumor mechanisms of Cremastra appendiculata polysaccharide (CAP) are not well understood.
- Hepatocellular carcinoma (HCC) remains a significant health concern with limited treatment options.
Purpose of the Study:
- To prepare and characterize CAP from Cremastra appendiculata.
- To investigate the anti-HCC activities of CAP.
- To elucidate the underlying mechanisms of CAP's antitumor effects.
Main Methods:
- CAP was prepared and structurally analyzed using techniques like GPC and NMR.
- In vitro assays were performed using Hepa1-6 cells and macrophage-conditioned medium.
- In vivo studies involved tumor-bearing mice treated with CAP, assessing tumor growth and immune responses.
Main Results:
- CAP is a linear glucomannan with a molecular weight of 8,058 Da.
- CAP-mediated macrophage-conditioned medium inhibited Hepa1-6 cell migration and proliferation.
- CAP treatment reduced tumor growth in mice, mitigated chemotherapy-induced organ damage, and enhanced immune parameters (TNF-α, IFN-γ).
Conclusions:
- CAP exhibits significant anti-HCC activity through direct tumor inhibition and immune system modulation.
- CAP demonstrates potential as a therapeutic agent for hepatocellular carcinoma.
- This study supports the development of natural polysaccharides from traditional medicine for cancer treatment.
