A coumarin-benzothiazole probe-driven multimodal aptasensing platform for label-free analysis and portable...

Kanamycin (KANA) is an aminoglycoside antibiotic that widely adopted in foods safety and the excessive intake can cause various symptoms. Although conventional methods for KANA detection have been well-developed, they still remain challenges in real scenarios application. We here report a coumarin-benzothiazole probe and KANA aptamer-based multimodal aptasensing platform (DR1/K-apt) to realize the efficient and accurate KANA analysis in milks. The formation of DR1/K-apt can severally produce the colorimetric/fluorescent/visual signals for KANA response within 30 min, which constitute the corresponded detection manners with sensitive detection limit (0.001 mM) and wide detection linear ranges. Especially, the fluorescence of DR1/K-apt can produce the obvious green-red-green colors variation and contribute to construct a smartphone-based portable paper device (K-cPAD) to reveal the facile quantification of KANA. It will hold the potential in realizing the large-scale instrument-free and chemical labelling-free KANA detection and offer a powerful tool in antibiotics evaluation and foods safety research.