Detection of human IgM and IgG antibodies reactive to Dengue virus using magnetic bead immunoassay

Here we describe a magnetic bead-based indirect ELISA that enables the rapid and scalable detection of human IgM and IgG antibodies reactive to dengue virus (DENV). The assay can be performed in 12 min in the chromogenic format to IgM or IgG detection. The system can also handle simultaneous detection of IgM and IgG within less than 8 min when combined with fluorescent-labelled secondary conjugated IgM and IgG detection antibodies. The sensitivity and specificity of the assay are in the same range as described for commercially available ELISA kits. This simple and ultrafast assay for dengue seroconversion detection is an efficient alternative for the rapid track dengue cases.