Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combined application of Bacillus velezensis JDB15 and Trichoderma harzianum JDL4 suppresses banana Fusarium wilt under controlled conditions.

Pest management science·2026

Same author

Three-port laparoscopic cholecystectomy with common bile duct exploration in situs inverse totalis: a case report and literature review.

Frontiers in surgery·2026

Same author

Development and validation of the Maladaptive Eating Behavior Questionnaire after Metabolic and Bariatric Surgery.

Obesity surgery·2026

Same author

Chemodivergent aminocarbonylation enabled by oxygen vacancy-engineered Pd-doped In<sub>2</sub>O<sub>3</sub> nanocatalysts.

Science advances·2026

Same author

Electron-Induced Reactions in Solid Carbon Dioxide: Lithography and Methane Formation under High-Energy Irradiation.

ACS applied materials & interfaces·2026

Same author

In Situ Generated Ru(III)/Ru(IV) From Ru(II) Catalyzed Aerobic Oxidation of Alcohols: Facile Access to Carboxylic Acids in Aqueous Media.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Ternary deep eutectic solvent (T-CuCl₂-EG) for lignin removal from corn straw.

Kang Zhao¹, Sha-Sha Zhao², Quan-Wei Liu¹

¹School of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China.

Journal of Biotechnology

|November 9, 2025

Summary

A novel deep eutectic solvent (DES) system efficiently removes lignin and hemicellulose from corn straw using microwave-assisted pretreatment, preserving valuable cellulose for biorefineries.

Keywords:

Corn straw Deep eutectic solvent Delignification Lewis acid Microwave-assisted

More Related Videos

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

Related Experiment Videos

Last Updated: May 1, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

Area of Science:

Biomass Valorization
Green Chemistry
Materials Science

Background:

Lignocellulosic biomass processing requires selective lignin removal to maximize cellulose utilization.
Conventional methods often struggle with lignin extraction efficiency and cellulose preservation.
Deep eutectic solvents (DES) offer tunable properties for biomass pretreatment.

Purpose of the Study:

To develop and optimize a novel ternary deep eutectic solvent (DES) for selective lignin removal from corn straw.
To investigate the synergistic effects of Lewis acid catalysis and hydrogen bond disruption in the DES system.
To achieve efficient biomass fractionation with high cellulose retention for biorefinery applications.

Main Methods:

Development of a ternary DES comprising triethylbenzyl ammonium chloride (T), copper chloride (CuCl₂), and ethylene glycol (EG).
Microwave-assisted pretreatment of corn straw using the optimized T-CuCl₂-EG DES.
Box-Behnken experimental design for optimizing pretreatment conditions (temperature, time, liquid-solid ratio, catalyst ratio).
Characterization of pretreated biomass using Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD).

Main Results:

Optimal delignification (96.52%) and hemicellulose removal (94.60%) achieved with 94.79% cellulose retention.
Optimized conditions: 130°C, 40 min, liquid-solid ratio 13.1, and CuCl₂/T molar ratio 0.198.
SEM, FT-IR, and XRD confirmed disruption of the lignocellulose matrix and selective solubilization of lignin/hemicellulose.
The T-CuCl₂-EG DES effectively broke ether/ester bonds in lignin-carbohydrate complexes.

Conclusions:

The novel ternary DES system demonstrates high efficiency in selective lignin and hemicellulose removal from corn straw.
Microwave-assisted pretreatment with T-CuCl₂-EG offers a sustainable and effective strategy for biomass fractionation.
Preservation of cellulose crystallinity indicates suitability for downstream biorefinery processes.