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Updated: Jan 11, 2026

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Green analytical method development and validation for Flavokawain A using RP-HPLC in bulk and in-house tablet dosage

Vaishnavi Pawar1, Vaibhav Kadam1, Bhatu Patil1

  • 1R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur District-Dhule, Maharashtra, 425405, India.

Annales Pharmaceutiques Francaises
|November 9, 2025
PubMed
Summary

A new, eco-friendly HPLC method accurately quantifies Flavokawain A, a kava compound with therapeutic potential. This validated technique ensures reliable quality control for Flavokawain A in bulk drugs and tablets.

Keywords:
Analyse pharmaceutiqueEco-friendly methodFlavokawain AFlavokawaïne AGreen RP-HPLCMethod validationMéthode éco-responsablePharmaceutical analysisPiper methysticumRP-HPLC verteValidation de méthode

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Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Green Chemistry

Background:

  • Flavokawain A, a chalcone from Piper methysticum (kava-kava), exhibits significant anti-inflammatory, analgesic, cardioprotective, and anticancer properties.
  • Despite its therapeutic potential, a validated analytical method for quantifying Flavokawain A is lacking.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and eco-friendly reverse-phase high-performance liquid chromatography (RP-HPLC) method.
  • To enable accurate quantification of Flavokawain A in bulk drug and tablet formulations.

Main Methods:

  • RP-HPLC separation using a Shim-pack GIST C18 column.
  • Methanol:water (85:15 v/v) mobile phase at 1.0 mL/min flow rate.
  • Method validation including linearity, sensitivity (LOD/LOQ), accuracy, and precision.

Main Results:

  • Flavokawain A eluted at 4.8 minutes with excellent linearity (R²=0.9999) over 2-12 μg/mL.
  • High sensitivity achieved with LOD of 0.281 μg/mL and LOQ of 0.853 μg/mL.
  • Accurate and precise quantification with recovery (99.2-101.3%) and low %RSD (<2%); assay results showed 100.1% in bulk and 99.5-101.7% in tablets.

Conclusions:

  • The developed RP-HPLC method is simple, accurate, precise, and environmentally sustainable (AGREE score 0.79).
  • This validated method is suitable for routine quality control and research-based quantification of Flavokawain A in pharmaceutical formulations.