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Related Experiment Video

Updated: May 3, 2026

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

Microglia Display Altered Spatial Morphology and Proteome After Stroke.

Brooke J Wanrooy¹, Jenny L Wilson¹, Althea R Suthya¹

¹Centre For Inflammatory Diseases, Department of Medicine, School of Clinical Sciences At Monash Health, Monash University, Victoria, Clayton, Australia.

|November 10, 2025

Summary

Ischaemic stroke alters the brain microenvironment, significantly impacting microglia morphology and function. These immune cells show region-specific changes, influencing inflammatory signaling pathways crucial for stroke recovery.

Keywords:

microglia morphology neuroinflammation proteome stroke

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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

Area of Science:

Neuroscience
Immunology
Stroke Research

Background:

Microglia are key immune cells in the central nervous system (CNS), essential for neural development and homeostasis.
They act as immune sentinels, constantly monitoring the neural environment and reacting to brain insults.

Purpose of the Study:

To investigate how the cerebral microenvironment, altered by ischaemic stroke, impacts microglial morphology and function in a spatially dependent manner.
To identify molecular changes in microglia following stroke.

Main Methods:

Transient middle cerebral artery occlusion (tMCAO) model in rodents.
Analysis of regional gene expression, microglial morphology via 3D reconstruction, and unbiased proteomics at 24 hours post-stroke.
Isolation of microglia from ipsilateral and contralateral hemispheres.

Main Results:

The infarct core exhibits a distinct proinflammatory microenvironment compared to sham controls.
Stroke induces region-specific microglial morphological changes, with amoeboid shapes and reduced complexity near the infarct.
108 differentially expressed proteins were identified in microglia, implicating pathways in cytokine production, chemokine activity, and leukocyte migration.

Conclusions:

Microglia morphology and function are significantly altered by ischaemic stroke in a region-specific manner.
These changes are linked to distinct inflammatory profiles and protein expression changes within the brain.
Microglia play critical roles in regulating inflammatory signaling post-stroke.