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Related Concept Videos

Drug Delivery: Overview01:16

Drug Delivery: Overview

708
The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
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Drug Delivery: Miscellaneous Routes01:22

Drug Delivery: Miscellaneous Routes

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Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection,  and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs...
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Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

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Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
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Modular Platform for Therapeutic Drug Delivery Using Trifunctional Bio-Orthogonal Macromolecular Conjugates.

Danmeng Luo1, Ning Wang1, Hannah Major-Monfried1,2

  • 1Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, United States.

Bioconjugate Chemistry
|November 10, 2025
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Summary
This summary is machine-generated.

Researchers developed a modular platform for creating versatile macromolecular conjugates (BMCs). This technology enables precise delivery of therapeutics, including gene editing tools, for various biomedical applications.

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Area of Science:

  • Bioconjugation Chemistry
  • Drug Delivery Systems
  • Molecular Medicine

Background:

  • Targeted delivery of macromolecular therapeutics offers advantages over small molecules for modulating protein-protein interactions and genome editing.
  • Efficient, safe, and cell-specific delivery of these large molecules remains a significant hurdle in therapeutic development.

Purpose of the Study:

  • To develop a modular platform for synthesizing heterotrifunctional bio-orthogonal macromolecular conjugates (BMCs).
  • To enable customizable and efficient delivery of diverse bioactive cargos for various biomedical applications.

Main Methods:

  • Engineered heterotrifunctional bio-orthogonal macromolecular conjugates (BMCs) by combining targeting ligands, cell-penetrating peptides (CPPs), and bioactive cargos.
  • Utilized facile bioconjugation chemistries to synthesize BMCs with enhanced yields, structural integrity, and activity.
  • Incorporated diverse components such as antibodies, receptor ligands, CPPs, optical probes, peptidomimetics, and CRISPR-Cas9 nuclease into BMCs.

Main Results:

  • Demonstrated BMCs' ability to facilitate endosomal escape and intracellular accumulation of fluorescently labeled cargo.
  • Showcased potent antileukemic activity of peptidomimetic MYB transcription factor inhibitor BMCs against acute myeloid leukemia cells.
  • Achieved rapid delivery and cell type-specific gene editing in human cells using CRISPR-Cas9 BMCs.

Conclusions:

  • The developed modular BMC platform allows for customizable delivery of functional macromolecules.
  • BMCs represent a broadly applicable platform with significant potential for advancing biomedical applications, including targeted therapy and gene editing.