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Updated: Jan 11, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
The protein-protein interaction between two spliced leader trans -splicing factors is mediated by two interlinked
Peter Eijlers1, Jonathan Pettitt1, Berndt Müller1
1School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, Scotland, United Kingdom.
Abstract:
SNA-1 and SNA-2 proteins are involved in spliced leader trans -splicing in Caenorhabditis elegans . They are components of the SL1 snRNP that donates the spliced leader 1 RNA which replaces the 5' end of most pre-mRNAs. SNA-1 and SNA-2 bind to each other, but the nature of their interaction is unclear. AlphaFold-Multimer predicts that the central region of SNA-1 and the C-terminal region of SNA-2 , each consisting of 3 α-helices, interlink to bring about the interaction between these proteins. Using yeast 2-hybrid assays we demonstrate that these regions are required and sufficient for this unusual mode of interaction between two proteins.
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