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Noncompetitive Fluorescence Polarization Aptamer Assay for Detecting SARS-CoV‑2 Variants.

Chang Yu-Ting1, Rigel Suzuki2, Yoichiro Fujioka3

  • 1Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.

ACS Omega
|November 10, 2025
PubMed
Summary
This summary is machine-generated.

A new noncompetitive fluorescence polarization aptamer assay (NC-FPAA) can rapidly detect multiple SARS-CoV-2 variants. This broad-spectrum diagnostic tool offers a simple, low-cost solution for effective viral surveillance.

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Virology

Background:

  • Emerging SARS-CoV-2 variants present ongoing public health challenges.
  • Current diagnostics often struggle with broad-spectrum detection due to viral mutations.
  • Rapid, cost-effective, and broadly applicable detection methods are crucial for surveillance.

Purpose of the Study:

  • To evaluate the noncompetitive fluorescence polarization aptamer assay (NC-FPAA) for detecting SARS-CoV-2, including variants.
  • To assess the assay's potential for broad-spectrum viral detection.
  • To determine if NC-FPAA can serve as a rapid, simple, and low-cost diagnostic tool.

Main Methods:

  • Utilized two fluorescently labeled aptamers (K1 and M40) with broad affinity for SARS-CoV-2 spike proteins.
  • Conducted detection experiments using eight different SARS-CoV-2 variants.
  • Assessed assay specificity against influenza viruses.

Main Results:

  • The NC-FPAA demonstrated applicability for detecting multiple SARS-CoV-2 variants.
  • The assay showed potential for broad-spectrum detection, unaffected by specific mutations.
  • Specificity was confirmed through testing with influenza viruses.

Conclusions:

  • NC-FPAA shows promise as a diagnostic tool for rapid, simple, and low-cost detection of multiple SARS-CoV-2 variants.
  • This assay can support broad and effective viral surveillance strategies.
  • The method is adaptable for detecting various viral strains, aiding in pandemic preparedness.