Optimization of forward extraction and functional properties of soy 11S globulin by reverse micelles with chaotropic...

Response surface methodology was employed to optimize the forward extraction of soy 11S globulin using sucrose-enhanced reverse micelles (RMs). Under optimal conditions (sucrose 0.012 mol/L, W₀, 18, pH 8.0, KCl 0.17 mol/L, 45 °C, 30 min, solid/liquid ratio 2.5 g/L), the extraction yield reached 76.07%. Compared with alkaline extraction-isoelectric precipitation, the RMs-sucrose method significantly improved protein purity (96.81%), solubility (100%), emulsifying activity, foaming properties, water absorption (2.80 g/g), and oil absorption (4.78 g/g). While surface hydrophobicity and emulsifying stability were reduced, the RMs-sucrose systems system preserved protein structure and modified surface properties, enabling superior functional performance. This approach demonstrates potential for scalable extraction of high-functionality soy 11S globulin.