A Novel CaCO₃/LPE/CA for Breeding Pyropia Haitanensis Artificial Shell-Boring Conchocelis

At present, shells have been used to incubate Pyropia haitanensis (P. haitanensis) shell-boring conchocelis. To develop a more convenient substrate for P. haitanensis conchocelis cultivation, a novel P. haitanensis shell-boring conchocelis culture substrate (CaCO₃/LPE/CA) was developed from calcite, low-density polyethylene, and calcium alginate. The mechanical properties and stability of CaCO₃/LPE/CA, including implanting density (ID) and Yield per unit area, were tested. The results showed that the CaCO₃/LPE/CA met practical P. haitanensis shell-boring conchocelis breeding application requirements. P. haitanensis was successfully cultured on CaCO₃/LPE/CA, and its life cycle was observed through biomicroscopy and scanning electron microscopy (SEM). After conchospores was succrssfully reseased from P. haitanensis conchosporangial branch, the movement and germination of conchospores were also observed. The yield of conchospores per unit area of optimized CaCO₃/LPE/CA reached 369.6 × 10³ conchospores/cm², making it a suitable candidate for P. haitanensis shell-boring conchocelis cultivation. Overall, CaCO₃/LPE/CA exhibits great potential as a marine bioactive material, and the process and cost of P. haitanensis culture breeding could be simplified due to its comprehensive characteristics.