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Updated: Jan 11, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Equisetum hyemale enhances local bone regeneration in murine maxillary defects: an in vivo study
Daniel Marques Leão1, Natália Aparecida Gomes1, Rosangela Maria Ferreira Costa E Silva2
1Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.
Abstract:
We investigated a maxillary bone defect model to test a synthetic extracellular matrix (HyStem®-HP) (hyaluronan) alone or associated with BG-Carb (BG-Carb)-a bioactive glass-carbon material made of a green waste (Equisetum hyemale). BG-Carb cytocompatibility was evaluated in Dental Pulp Cells (DPC) at 24, 48, and 72 h. Animals were treated with both materials and euthanized on days 7 and 21. The inflammatory process, bone regeneration, and epithelial formation were quantified. Data were analyzed at a 95% confidence level using Student's t-test or Mann Whitney test (p < 0.05). BG-Carb did not impair dental pulp cell viability. BG-Carb also promoted increased bone formation at 7 days compared to HyStem®-HP alone. At 21 days, bone formation was similar between groups; however, less granulation tissue and greater epithelial thickness were observed in the BG-Carb-treated samples. BG-Carb showed biocompatibility promoting early bone regeneration and tissue organization during repair. BG-Carb is a promise candidate for bone regeneration.
