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Published on: July 10, 2014
Push-out bond strength of biodentine after calcium hydroxide removal using novel chelation-activation protocols in
Arzu Kaya Mumcu1, Arzu Şahin Mantı2
1Department of Endodontics, Faculty of Dentistry, Kutahya Health Sciences University, 43100, Kutahya, Turkey. arzu.kayamumcu@ksbu.edu.tr.
Abstract:
This study evaluated the effects of three chelating agents-glycolic acid (GA), ethylenediaminetetraacetic acid (EDTA), and etidronic acid (HEDP)-combined with different irrigation activation techniques on the pushout bond strength of Biodentine to root dentin after calcium hydroxide removal. A total of 120 extracted single-rooted mandibular premolars were randomly assigned to nine experimental and one control group (n = 12). Following Ca(OH)2 dressing and incubation, canals were irrigated with GA, EDTA, or HEDP, each activated using passive ultrasonic irrigation (PUI), XP-Endo Finisher (XPF), or conventional needle irrigation (CNI). Biodentine was placed into 2 mm-thick root slices, and pushout bond strength was measured using a universal testing machine. Statistical analysis was performed using one-way ANOVA for group comparisons, and two-way ANOVA was used to evaluate factor effects (α = 0.05). Overall, GA produced higher bond strength than EDTA (p = 0.027). The difference between GA and HEDP was not significant under PUI activation; however, GA yielded significantly higher bond strength than HEDP when activated with CNI or XPF. Using 10% GA with PUI enhanced Biodentine-dentin adhesion, suggesting its potential as a biocompatible and effective alternative to conventional chelators in regenerative endodontic treatments, improving coronal sealing and long-term stability.
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