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Updated: Jan 11, 2026
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Published on: November 1, 2024
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Methods for detecting off-target effects of CRISPR/Cas9.
Ying-Ying Xu1, Sheng-Mei Zhou2, Lu-Yan Wang2
1Laboratory Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.; Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Biotechnology Advances
|November 10, 2025
Summary
The CRISPR/Cas9 gene editing tool can cause unintended mutations. This paper reviews methods to detect and evaluate these off-target effects, improving CRISPR accuracy and safety in research and therapy.
Area of Science:
- Biotechnology
- Genomics
- Molecular Biology
Background:
- CRISPR/Cas9 is a powerful gene editing technology known for its simplicity and efficiency.
- Off-target mutations induced by CRISPR/Cas9 can reduce the precision of gene modifications.
Purpose of the Study:
- To provide an overview of methodologies for identifying CRISPR/Cas9 off-target effects.
- To offer insights for enhancing the precision and safety of CRISPR applications.
Main Methods:
- Review of current and emerging strategies for detecting CRISPR/Cas9 off-target mutations.
- Analysis of techniques for evaluating the impact of unintended genetic alterations.
Main Results:
- CRISPR/Cas9 necessitates robust methods for off-target analysis.
- Various techniques exist for predicting, detecting, and evaluating off-target mutations.
Conclusions:
- Accurate identification of off-target effects is vital for reliable CRISPR/Cas9 gene editing.
- Improved detection strategies will enhance the safety and therapeutic potential of CRISPR technology.
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