Related Experiment Video
Updated: Jan 11, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM
Published on: August 5, 2009
A convolutional neural network-based framework for quality control through speckle displacement analysis
Hamed Sabahno1, Davood Khodadad2
1Department of Applied Physics and Electronics, Umeå University, Umeå, 90187, Sweden. hamed.sabahno@stat.lu.se.
None:
Among the most advanced techniques for quality control, image processing and optical methods are prominent because of their precision and versatility. These methods often involve analyzing speckles generated by coherent laser illumination because coherent light provides detailed and accurate measurement capabilities. In speckle metrology-based techniques, the accurate measurement of speckle displacements is crucial for detecting faults or deformations in objects. In this study, an advanced algorithm segments the image into overlapping grids, followed by a Fourier-based image registration to accurately quantify the speckle displacements. This method can simultaneously detect multiple translational movements in the different parts of an object. However, proper calculation and assignment of overlap sizes to each grid plays a crucial role in this method, which is where we obtain help from convolutional neural networks (CNNs). We develop a CNN architecture and optimize its hyperparameters using a Monte Carlo simulation algorithm incorporating a grid search and k-fold cross-validation. Finally, we validate the developed method through a case study involving a simulation and real speckle patterns generated by spraying water on a cardboard surface.
Related Concept Videos
Deconvolution
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

