Therapeutic potential of Bothrops leucurus phospholipase A₂ in preventing dengue virus infection

BlD-PLA₂, a phospholipase A₂ isolated from Bothrops leucurus venom, exhibited notable antiviral activity against dengue virus (DENV) in vitro. Treatment significantly reduced viral RNA levels, particularly when administered during the infection period, suggesting interference with early stages of viral entry. Immunofluorescence and ELISA assays confirmed its interaction with host cell components. Importantly, reseeding assays demonstrated that residual viral RNA detected after treatment was not associated with infectious particles. These findings indicate that BlD-PLA₂ effectively disrupts DENV infection and support its potential as a lead compound for the development of novel antiviral strategies.