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¹³C NMR: ¹H–¹³C Decoupling01:04

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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NMR Spectrometers: Overview01:20

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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¹H NMR Signal Integration: Overview00:58

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The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
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Carbon-13 (¹³C) NMR: Overview01:10

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Carbon-13 is a naturally occurring NMR-active isotope of carbon with a low natural abundance of 1.1%. In contrast, carbon-12 is the most abundant isotope of carbon with zero nuclear spin. Therefore, it is NMR inactive. The gyromagnetic ratio of carbon-13 is smaller than that of protons. As a result, carbon-13 resonance is about 6000 times weaker than proton resonance. For a given magnetic field strength, the resonance frequency of carbon-13 is about one-fourth of the resonance frequency for...
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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
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Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
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30-Channel Microcoil System for High-Throughput Metabolic Flux Analysis Using Hyperpolarized 13C NMR Spectroscopy.

Nichlas Vous Christensen1, Sissel Ellemose Liboriussen1, Juan D Sanchez-Heredia2,3

  • 1The MR Research Centre, Aarhus University, Aarhus, Denmark.

NMR in Biomedicine
|November 10, 2025
PubMed
Summary
This summary is machine-generated.

A new 30-channel microcoil array enables high-throughput metabolic flux measurements using hyperpolarized (HP) tracers. This cost-effective system achieves high statistical power for studying diseases like cancer, advancing translational research.

Keywords:
carbon‐13cellshigh‐throughputhyperpolarizationmetabolismmultichannel

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Area of Science:

  • Metabolic research
  • Biophysics
  • Medical imaging

Background:

  • Hyperpolarized (HP) carbon-13 labeled compounds offer real-time metabolic tracking in vivo and ex vivo.
  • Current HP techniques are limited by high cost and complexity, hindering widespread adoption in translational research.
  • There is a need for cost-effective, high-throughput methods to enhance the utility of HP tracers.

Purpose of the Study:

  • To develop a high-throughput system for simultaneous metabolic flux measurements using HP tracers.
  • To demonstrate the system's capability in detecting metabolic changes in cancer cells.
  • To establish a new benchmark for high-throughput HP technologies.

Main Methods:

  • A 30-channel microcoil receiver array was designed for simultaneous measurements.
  • Metabolic flux was assessed using a single dissolution of HP [1-13C]pyruvate.
  • Pyruvate-to-lactate conversion was measured in acute myeloblastic leukemia ML-1 cells treated with 2-deoxy-d-glucose.

Main Results:

  • The 30-channel system significantly increased throughput for metabolic flux measurements.
  • Significant changes in pyruvate-to-lactate conversion were detected in treated leukemia cells (p < 0.001).
  • High statistical power was achieved per HP pyruvate dissolution, despite perfusion variability.

Conclusions:

  • The developed microcoil array establishes a new benchmark for high-throughput HP metabolic research.
  • This technology has potential for drug screening, disease modeling, and studying metabolic disorders.
  • The modular design is adaptable for various sample types, supporting diverse translational research applications.