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Related Experiment Videos

Technique for differentiating particles that are cell-associated or ingested by macrophages.

D E Gardner, J A Graham, F J Miller

    Applied Microbiology
    |March 1, 1973
    PubMed
    Summary

    A new xylene-based method accurately distinguishes particle attachment from ingestion during phagocytosis. This technique enhances the measurement of phagocytic activity by quantifying both attached and ingested particles.

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    Area of Science:

    • Cell Biology
    • Immunology
    • Microscopy Techniques

    Background:

    • Phagocytosis is a critical cellular process for particle clearance and immune response.
    • Distinguishing particle attachment from true ingestion under light microscopy can be challenging.
    • Existing methods may not precisely quantify the extent of phagocytic uptake.

    Purpose of the Study:

    • To develop and validate a simple, accurate method for differentiating particle attachment from ingestion in phagocytosis.
    • To improve the quantitative assessment of phagocytic activity.
    • To provide researchers with a reliable technique for analyzing phagocytosis.

    Main Methods:

    • Utilized polystyrene latex spheres as model particulate material.
    • Applied a novel xylene treatment to selectively dissolve extracellular spheres.

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  • Employed light microscopy to count remaining intracellular spheres for phagocytic index determination.

    • Main Results:

    • The xylene treatment effectively removed extracellular spheres, allowing clear visualization of ingested particles.
    • Accurate phagocytic, ingestion, and attachment indices were obtained.
    • The method proved to be easy to perform and highly accurate.

    Conclusions:

    • Xylene treatment offers a superior approach for quantifying phagocytosis compared to traditional methods.
    • This technique provides distinct measures of particle attachment and ingestion.
    • The method facilitates more precise evaluation of cellular phagocytic capacity.