Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Obtaining Topography01:25

Methods of Obtaining Topography

270
Topography involves measuring and mapping land elevations, natural features, and artificial structures to create accurate representations of the terrain. Topographic surveying relies on traditional and modern methods, each with distinct advantages and limitations.Traditional Surveying Methods:Transit stadia surveys and plane table surveys were widely used traditional surveying methods. These techniques relied on instruments like theodolites and stadia rods for measuring distances and angles,...
270
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

750
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
750
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drug repurposing for skin pigmentation disorders: Discovery of novel tyrosinase inhibitors.

Bioorganic chemistry·2026
Same author

Dendrimers in cancer therapy: emerging clinical design strategies.

Trends in pharmacological sciences·2026
Same author

Epidemiological and Genomic Characterization of Japanese Encephalitis Virus in Mosquitoes in China.

Journal of medical virology·2026
Same author

Morphological characterization of floral ontogeny in Lagerstroemia indica.

BMC plant biology·2026
Same author

Association between obstructive sleep apnea, high-sensitivity c-reactive protein, and mitral annular calcification: a cross-sectional analysis of 200 adults undergoing routine medical evaluation.

Molecular and cellular biochemistry·2026
Same author

Caveolin 1 has a comparable role as endothelial progenitor cells to repair vascular endothelial dysfunction caused by the combined exposure to arsenic and lipid.

The Journal of nutritional biochemistry·2026

Related Experiment Video

Updated: Jan 11, 2026

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
10:14

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Published on: May 12, 2019

7.6K

Three-dimensional topography reconstruction method for non-uniform under-sampled vertical scanning

Junhao Kuang, Zhenyan Guo, Zhishan Gao

    Optics Express
    |November 11, 2025
    PubMed
    Summary

    This study introduces an envelope centroid localization method (ECS) for low-coherence scanning interferometry (LCSI). ECS significantly enhances 3D surface topography measurement accuracy and efficiency, especially with sparse and non-uniform sampling.

    More Related Videos

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    10.5K
    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
    09:56

    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

    Published on: August 21, 2019

    7.3K

    Related Experiment Videos

    Last Updated: Jan 11, 2026

    3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
    10:14

    3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

    Published on: May 12, 2019

    7.6K
    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    10.5K
    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
    09:56

    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

    Published on: August 21, 2019

    7.3K

    Area of Science:

    • Metrology
    • Optical Engineering
    • Surface Science

    Background:

    • Low-coherence scanning interferometry (LCSI) offers high-precision 3D surface topography measurement.
    • Existing LCSI methods face limitations in accuracy and efficiency due to non-uniform sampling and large sampling intervals.

    Purpose of the Study:

    • To develop a novel method for improving LCSI measurement accuracy and efficiency.
    • To address challenges posed by sparse and non-uniform sampling in LCSI data acquisition.

    Main Methods:

    • Proposed an envelope centroid localization (ECS) method utilizing sinc function interpolation.
    • Exploited the similarity between sinc function and interference signal distribution for interpolation.
    • Compensated for non-uniform and sparse sampling in interference envelope characteristic regions.

    Main Results:

    • Achieved over 300% improvement in measurement accuracy compared to traditional Center of Mass (COM) and Peak Fitting (PF) methods.
    • Maintained reconstructed surface waviness around 0.05 µm under extreme sparse sampling (17x Nyquist interval).
    • Demonstrated effectiveness with non-uniform sampling intervals (difference up to ±0.2 µm).

    Conclusions:

    • The ECS method effectively mitigates information loss from insufficient and variable sampling intervals.
    • ECS provides a superior balance between measurement accuracy and efficiency in LCSI.
    • Validated the method's efficacy for processing challenging sparse and non-uniform sampling data in LCSI.