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Related Experiment Video

Updated: Jan 11, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
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Phylogenomic Analyses and DNA Barcoding Development Within Moraceae: Insights Into Genomic Features, Mutational

Li-Na Zhou1, Shi-Zhen Wu1, Qing Ma2

  • 1College of Life and Environmental Science Wenzhou University Wenzhou China.

Ecology and Evolution
|November 11, 2025
PubMed
Summary

This study resolves the Moraceae family phylogeny using plastome data, revealing tribal monophyly and ancient divergence. These findings offer a framework for revising taxonomy and exploring genetic resources in this valuable plant family.

Keywords:
Moraceaemolecular markersphylogenyplastomepositive selection

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Area of Science:

  • Botany
  • Genomics
  • Evolutionary Biology

Background:

  • Moraceae, a diverse family with 1200 species, holds significant value but faces taxonomic challenges due to morphological variability.
  • Understanding the phylogeny of Moraceae is crucial for its effective utilization in food, medicine, and industry.

Purpose of the Study:

  • To resolve the tribal-level phylogeny of the Moraceae family using complete plastome sequences.
  • To identify molecular markers and investigate evolutionary patterns within Moraceae.

Main Methods:

  • Sequencing of seven complete Moraceae plastomes and analysis of existing data for 46 species.
  • Comparative plastome analysis, identification of repeats and simple sequence repeats (SSRs).
  • Phylogenetic analysis, evolutionary rate estimation, and positive selection detection on specific genes.

Main Results:

  • Plastomes exhibit a typical quadripartite structure (158,459–162,594 bp) with 10 highly variable regions identified.
  • Over 5350 dispersed/tandem repeats and 4751 SSRs were found, indicating potential for molecular marker development.
  • Moraceae tribes are confirmed as monophyletic, diverging approximately 79.43 million years ago, coinciding with Cretaceous angiosperm diversification.

Conclusions:

  • The study provides a robust phylogenetic framework for the Moraceae family, aiding taxonomic revisions.
  • Genomic resources generated will support future genetic engineering and germplasm exploration within Moraceae.