Shared Biomarkers and Potential Mechanisms Between Hashimoto's Thyroiditis and Recurrent Miscarriage Revealed by...

Area of Science:

• Endocrinology and Immunology
• Reproductive Medicine
• Bioinformatics

Background:

• Hashimoto's thyroiditis (HT) is increasingly linked to recurrent miscarriage (RM), but underlying mechanisms remain unclear.
• Identifying shared biomarkers is crucial for understanding the association between HT and RM.
• This research focuses on elucidating the molecular and immunological connections between these two conditions.

Purpose of the Study:

• To identify key shared biomarkers between Hashimoto's thyroiditis and recurrent miscarriage using bioinformatics.
• To reveal potential molecular mechanisms and immune microenvironment characteristics associated with these shared biomarkers.
• To provide a theoretical basis and potential targets for diagnosing and treating the link between HT and RM.

Main Methods:

• Integrated transcriptomic analysis of HT thyroid and RM endometrial tissue datasets from the Gene Expression Omnibus (GEO) database.
• Identification of differentially expressed genes (DEGs) and candidate biomarkers through protein-protein interaction (PPI) network analysis.
• Gene Set Enrichment Analysis (GSEA) for pathway analysis and ssGSEA for immune cell infiltration quantification, followed by molecular docking for drug prediction.

Main Results:

• Cofilin-1 (CFL1) and TRAPPC1 were identified as significant biomarkers, upregulated in both HT and RM.
• Biomarkers were enriched in pathways including "autoimmune thyroid disease" and "antigen processing and presentation".
• Significant differences in monocyte and eosinophil infiltration were observed, correlating with biomarker expression, and potential therapeutic drugs were predicted.

Conclusions:

• CFL1 and TRAPPC1 serve as common biomarkers for Hashimoto's thyroiditis and recurrent miscarriage.
• These findings offer novel insights for fundamental research and early clinical diagnosis and treatment strategies.
• The study provides a foundation for further investigation into the pathogenesis and therapeutic interventions for the HT-RM association.