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Updated: Jan 11, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
Epstein-Barr Virus Expressed Long Non-Coding RNA (lncBARTs) Regulate EBV Latent Genome Replication
Jiayan Liu1,2, Dittman Lai-Shun Chung1,2,3, Larry Ka-Yue Chow3
1State Key Laboratory for Emerging Infectious Diseases and Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
Abstract:
Epstein-Barr virus (EBV) is a ubiquitous human virus that is also linked to various human cancers. In EBV-associated nasopharyngeal carcinoma and gastric carcinoma cells, EBV expresses only essential viral antigens but high levels of long non-coding RNAs known as BamHI-A rightward transcripts (lncBARTs). The exact roles lncBARTs in the EBV life cycle and the development of EBV-associated cancers are largely not understood. This study demonstrates that lncBARTs play a role in maintaining viral genome replication by affecting the tethering of EBV oriP region to chromosome. Mechanistically, lncBARTs interact functionally with a complex consisting of Bromodomain-containing protein 4 (BRD4), CCTC-binding factor (CTCF), and EBV nuclear antigen 1 (EBNA1), anchoring their binding at oriP to facilitate EBV episome replication. Additionally, the lncBARTs-BRD4/CTCF complex contributes to the regulation of MYC and BCL2 expression. These findings suggest that lncBARTs modulate EBV latency through interactions with oriP region, while lncBARTs-BRD4/CTCF complexes promote host epigenome reprogramming and drive tumorigenesis in EBV-associated epithelial tumors.
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