Related Experiment Video
Updated: Jan 6, 2026

Author Spotlight: Enhancing Skin Model Diversity with Cost-Effective 3D Cellular Models
Published on: October 20, 2023
Biometric Quantification of Keratinocyte Turnover Using Bioengineered Human Skin Equivalents
Georgia Abraham1, Julia Maczkowska1, Rachel Gilmore1
1Department of Biosciences, Durham University, Durham, UK.
None:
Effective epidermal cell turnover is essential for the formation and maintenance of the epidermis. Dysregulation of keratinocyte turnover impedes skin barrier integrity and contributes to disease pathophysiology. Full-thickness human skin equivalents (FT-HSE) offer a physiologically relevant in vitro platform for studying skin biology, providing valuable insights into the epidermal response to both endogenous and exogenous stimuli. This chapter describes immunofluorescent staining of Ki67 and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay for assessing cellular proliferation and apoptosis, thereby providing insights into keratinocyte turnover within FT-HSEs.
Related Concept Videos
Renewal of Skin Epidermal Stem Cells
Cells of the Epidermis
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Clinical Applications of Epidermal Stem Cells

