Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.5K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.5K
RNA Interference01:23

RNA Interference

27.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.7K
Experimental RNAi02:15

Experimental RNAi

7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.6K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.6K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.3K
Types of RNA01:23

Types of RNA

72.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic and Proteomic Insights Into Diapause in the Wheat Stink Bug, Aelia rostrata.

Archives of insect biochemistry and physiology·2026
Same author

Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.

Insect molecular biology·2026
Same author

Proteomic changes associated with the initiation and termination of aestivation in the cabbage stem flea beetle.

Journal of insect physiology·2026
Same author

Insect Lipidomics: Advances, Applications, and Physiological Insights.

Advances in experimental medicine and biology·2026
Same author

Lipid Metabolism in Diapause.

Advances in experimental medicine and biology·2026
Same author

Orthologous genes of the red flour beetle Tribolium castaneum and the vinegar fly Drosophila melanogaster.

BMC genomic data·2025

Related Experiment Video

Updated: Jan 11, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.7K

Small RNAs in insects: emerging classes and functions.

Doga Cedden1, Gözde Güney2

  • 1Department of Evolutionary Developmental Genetics, Göttingen Center for Molecular Biosciences, University of Göttingen, Johann-Friedrich-Blumenbach Institute, Göttingen, Germany.

Current Opinion in Insect Science
|November 11, 2025
PubMed
Summary

Insect small RNAs (sRNAs) regulate genes and immunity. Emerging research reveals their roles in development, pest control, and cross-kingdom communication, offering novel pest management strategies.

More Related Videos

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

11.3K
RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

8.2K

Related Experiment Videos

Last Updated: Jan 11, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.7K
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

11.3K
RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

8.2K

Area of Science:

  • Entomology and Molecular Biology
  • Focuses on insect small RNA (sRNA) biology and its applications.

Background:

  • Insects utilize diverse sRNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs), for gene regulation and immunity.
  • Transfer RNA-derived sRNAs (tsRNAs) are emerging as critical players in insect development and stress responses.

Purpose of the Study:

  • To review recent advances in insect sRNA biology, covering canonical and emerging classes.
  • To explore the functional roles of insect sRNAs in gene regulation, immunity, development, reproduction, and cross-kingdom interactions.
  • To highlight the potential of insect sRNAs for developing novel pest management strategies.

Main Methods:

  • Literature review of recent advances in insect sRNA biology.
  • Summary of canonical sRNA classes (miRNAs, siRNAs, piRNAs) and emerging tsRNAs.
  • Discussion of methodologies like RNA sequencing and degradomics for mechanistic studies.

Main Results:

  • Mechanistic studies are increasingly linking sRNAs to biological phenomena.
  • siRNAs show potential for pest control via double-stranded RNA (dsRNA) technology, with piRNAs crucial for transposon control.
  • Emerging roles for piRNAs in reproduction and sex determination, and cross-kingdom sRNA exchange between insects and plants were identified.

Conclusions:

  • Insect sRNA biology is rapidly expanding, revealing diverse functions.
  • Understanding insect sRNAs provides a foundation for innovative and species-specific pest control strategies.
  • Cross-kingdom sRNA interactions highlight complex ecological relationships with potential biotechnological applications.