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Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
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Reference Values for Extended Lymphocyte Subsets in Korean Children: A Multicenter Study Using the EuroFlow PIDOT

Kyung-Hwa Shin1,2, Hyun-Woo Choi3, Jihyang Lim4

  • 1Department of Laboratory Medicine, Pusan National University School of Medicine, Busan, Korea.

Annals of Laboratory Medicine
|November 12, 2025
PubMed
Summary
This summary is machine-generated.

This study establishes reference values for lymphocyte subpopulations in Korean children, revealing age and sex differences in immune cell counts. These findings are crucial for diagnosing immune disorders and guiding pediatric immunotherapy.

Keywords:
B-lymphocyte subsetsLymphocyte subsetsNatural killer cellsT-lymphocyte subsets

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Area of Science:

  • Immunology
  • Pediatric Hematology

Background:

  • Reference intervals for lymphocyte subpopulations are limited for Korean children, especially for extended subsets.
  • Existing data primarily originates from Western populations.

Purpose of the Study:

  • To determine absolute cell counts and percentages of lymphocyte subpopulations in Korean children.
  • To establish age- and sex-specific reference values for lymphocyte subsets in this population.

Main Methods:

  • Collected samples from 92 Korean children aged 5-17 years, stratified into two age groups.
  • Utilized flow cytometry with the Primary Immunodeficiency Orientation Tube (PIDOT) panel for immunophenotyping.
  • Classified cells into T, B, and natural killer populations, with further subdivision of T and B cells by maturation and specific markers (e.g., CD4+, CD8+).

Main Results:

  • Younger children (5-9 yrs) had higher counts of total B cells, unswitched memory B cells/plasma cells, total T cells, CD4+ naïve cells, and TCRγδ+ T cells compared to older children (10-17 yrs).
  • Older children exhibited higher CD4+ effector memory (EM) T cells.
  • Sex-based differences were observed, with males showing higher total B cells and CD4+ EM cells, while females had higher proportions of CD4+ and CD4+ naïve T cells.

Conclusions:

  • This study provides the first reference values for extended lymphocyte subsets in Korean children using the PIDOT panel.
  • Lymphocyte subset distributions are influenced by age, sex, and laboratory factors.
  • The established reference data can aid in the diagnosis of immune disorders and the application of immunotherapy in pediatric patients.