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Effector-Mediated Spatial Reprogramming of Glycolate Oxidase Subverts Peroxisomal and Membrane-Associated ROS
Junjian Situ1, Zijing Zhang1, Yi Shao1
1National Key Laboratory of Green Pesticide/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
Plant Biotechnology Journal
|November 12, 2025
Summary
The Peronophythora litchii effector PlAvh133 suppresses plant immunity by targeting glycolate oxidase (GLO) LcGLO1. This oomycete effector relocates LcGLO1 to the plasma membrane, inhibiting its activity and reducing reactive oxygen species (ROS) production.
Area of Science:
- Plant Pathology
- Molecular Plant-Microbe Interactions
- Biochemistry
Background:
- Oomycete pathogens employ RXLR effectors to suppress host immunity.
- Glycolate oxidase (GLO) is a key enzyme in photorespiration with a role in plant defense.
Purpose of the Study:
- To investigate the virulence mechanism of the Peronophythora litchii RXLR effector PlAvh133.
- To determine the interaction between PlAvh133 and the litchi glycolate oxidase (GLO) LcGLO1 and its impact on plant immunity.
Main Methods:
- Investigated effector-target interaction using molecular biology techniques.
- Assessed effector localization and function in planta.
- Analyzed the role of LcGLO1 in plant resistance and susceptibility to oomycete pathogens.
Main Results:
- PlAvh133 targets and binds to litchi GLO LcGLO1, acting as a virulence factor.
- PlAvh133 relocates LcGLO1 from peroxisomes to the plasma membrane, inhibiting its enzymatic activity.
- This effector-mediated process suppresses reactive oxygen species (ROS) burst by interfering with LcGLO1, LcCATB, LcRBOHD, and LcCPK5 interactions.
Conclusions:
- PlAvh133 employs an novel strategy to suppress plant immunity by manipulating host GLO1.
- Pathogen effectors can reprogram host cellular compartments and enzymatic functions to promote virulence.
- Understanding this mechanism provides insights into oomycete pathogenesis and potential targets for disease control.
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