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Updated: Jan 6, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
5mC and 5hmC methylation sequencing: the power of 6-base sequencing in a multiomic era
Robert Crawford1, Tom Charlesworth1, Stephen Riffle2
1biomodal Ltd, Chesterford Research Park, Saffron Walden, UK.
Abstract:
The integration of genetic and epigenetic information is essential for a comprehensive understanding of genome function and regulation. Traditional sequencing methods often fall short in capturing both genetic variants and epigenetic modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) simultaneously. Recent advances in 6-base sequencing have enabled the simultaneous, base-resolution detection of canonical bases and key cytosine modifications in a single workflow. This review explores the biological significance of 5mC and 5hmC, discusses current methods to achieve 6-base sequencing, and highlights recent applications in academic and clinical settings.
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