Related Experiment Video
Updated: Jan 11, 2026
07:24
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
Published on: July 9, 2021
2.6K
Long Non-Coding RNA NEAT1 Inhibits Enterovirus 71 Replication by Enhancing IFN-β Transcription Through DDX60
Dong Li1, Shuaiyin Chen1, Wangquan Ji1
1Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, China.
The Journal of Infectious Diseases
|November 12, 2025
Summary
Enterovirus 71 (EV71) infection increases NEAT1, which enhances interferon-beta (IFN-β) production to fight the virus. Lower NEAT1 levels in hand, foot, and mouth disease (HFMD) patients indicate a poorer prognosis.
Area of Science:
- Molecular Biology
- Immunology
- Virology
Background:
- Enterovirus 71 (EV71) causes severe hand, foot, and mouth disease (HFMD), but its pathogenesis is unclear.
- Understanding host responses to EV71 is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the role of NEAT1 in EV71 infection and host defense.
- To elucidate the molecular mechanisms by which NEAT1 influences EV71 pathogenesis.
Main Methods:
- EV71 infection models in vitro and in vivo.
- Analysis of NEAT1 and paraspeckle formation.
- Investigation of the DDX60-IRF7 pathway and IFN-β transcription.
- Case-control study correlating NEAT1 levels with HFMD severity.
Main Results:
- EV71 infection upregulates NEAT1, leading to paraspeckle formation.
- NEAT1 enhances IFN-β transcription via the DDX60-IRF7 pathway, promoting anti-EV71 resistance.
- NEAT1 facilitates SFPQ relocation, relieving transcriptional repression on DDX60 and MDA5.
- Reduced NEAT1-2 expression in HFMD patients correlates with disease severity.
Conclusions:
- NEAT1 acts as an intrinsic antiviral molecule against EV71.
- NEAT1-mediated IFN-β production is a key host defense mechanism against EV71.
- Low NEAT1 levels may predict a poor prognosis in HFMD patients.
Related Concept Videos
01:29Viruses with RNA Genomes
774
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
774
02:39lncRNA - Long Non-coding RNAs
9.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
01:23RNA Interference
27.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.7K
01:20Types of RNA
9.0K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
9.0K
02:28Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K
03:18Non-LTR Retrotransposons
13.1K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.1K