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Updated: Jan 11, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Comparing two Integrated Fixed-film Activated Sludge (IFAS) systems in view of the updated European Urban Wastewater
Antonio Mineo1, Dilsad Soylu2, Jie Jiang3
1Engineering Department, Palermo University, Viale delle Scienze, Ed. 8, 90128, Palermo, Italy.
Abstract:
This study evaluates the pollutant removal performances and environmental impacts of two pilot-scale configurations: (I) an integrated fixed-film activated sludge (IFAS) system combined with a membrane bioreactor (MBR), and (II) IFAS coupled with secondary settling followed by ultrafiltration (UF). Treatment performance, membrane fouling, microbial activity (as measured by respirometry), nitrous oxide (N2O) emissions, and carbon footprint (CF) were evaluated. Both systems achieved over 97 % total chemical oxygen demand removal, with slightly higher ammonium nitrogen removal in Configuration I (96 ± 3 % vs. 93 ± 6 %). Under the revised UWTD (2024/3019), effluent PO43--P averaged about 5 mg L-1 for both configurations, exceeding the 1 mg L-1 discharge limit. For reuse (Regulation (EU) 2020/741), both configurations consistently met the Class B BOD₅ standard but failed to consistently achieve the more stringent Class A standard. All other monitored parameters complied with regulations. Respirometry tests indicated higher heterotrophic activity in the suspended biomass of Configuration II. Configuration I achieved superior total nitrogen removal (85 ± 11 % vs. 75 ± 15 %) due to its higher internal carbon-to‑nitrogen ratio (4.2 vs. 2.1 gCOD/gN). Membrane fouling was more pronounced in the MBR system, with an average fouling rate of 0.21 ± 0.2 bar day-1 (0.16 ± 0.2 bar day-1). N2O emissions remained low, with emission factors slightly lower in the MBR (0.13 %) compared to the Settler-UF (0.09 %). The carbon footprint was estimated at 1.47 kg CO2eq d-1 for Configuration I and 2.27 kg CO2eq d-1 for Configuration II, mainly reflecting differences in energy consumption and nitrogen removal performance. Overall, combining IFAS with membrane technologies provides clear advantages for producing high-quality effluent, meeting regulatory requirements, and reducing greenhouse gas emissions.

