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Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Related Experiment Video

Updated: Jan 11, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Accurate quantification of human miRNA isoforms using the PROMER technology.

Young-Hyean Nam1, Taeho Kwak2, Saemi Jeon2

  • 1NuriBio Co., Ltd, Anyang-si, Gyeonggi-Do, 14058, Republic of Korea; College of Pharmacy, Kangwon National University, 1 Kangwondaehakgil, Chuncheon, 24341, Republic of Korea.

Biochemical and Biophysical Research Communications
|November 12, 2025
PubMed
Summary

MicroRNAs (miRNAs) and their variants, isomiRs, regulate gene expression. We developed a new qRT-PCR technique to distinguish isomiRs, crucial for understanding disease and developing therapies.

Keywords:
PROMERRNAse H2miRNAmiRNA isoform

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

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327

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional gene regulators.
  • IsomiRs, miRNA sequence variants, exhibit differential expression in development and disease.
  • Understanding isomiR diversity is vital for miRNA-based diagnostics and therapeutics.

Purpose of the Study:

  • To develop an innovative qRT-PCR technique for distinguishing isomiRs.
  • To build upon the previously reported PROMER technology for enhanced miRNA analysis.

Main Methods:

  • Utilized a quantitative reverse transcription PCR (qRT-PCR) approach.
  • Integrated the PROMER technology involving RNA:DNA matching and RNase H2 cleavage.
  • Developed a novel method for precise isomiR differentiation.

Main Results:

  • Successfully established a qRT-PCR-based technique to differentiate various isomiRs.
  • The method builds upon the principles of PROMER technology for accurate measurement.
  • Demonstrated the capability to distinguish sequence variants of miRNAs.

Conclusions:

  • The developed qRT-PCR technique offers a novel approach for isomiR analysis.
  • This advancement is critical for studying miRNA function and disease mechanisms.
  • Facilitates the development of more precise miRNA-based diagnostics and therapeutics.