Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Monitoring systemic immune responses to checkpoint inhibition in breast cancer reveals host responses and mechanisms of resistance.

Journal for immunotherapy of cancer·2025

Same author

Clonal Hematopoiesis of Indeterminate Potential Influences Breast Cancer Outcomes in a Genotype-Specific Manner.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Same author

Tumor cell-adipocyte gap junctions activate lipolysis and contribute to breast tumorigenesis.

Nature communications·2025

Same author

Phase Ib Trial of Fulvestrant, Palbociclib, and Erdafitinib, a pan-FGFR Tyrosine Kinase Inhibitor, in HR+/HER2- Metastatic Breast Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Same author

A phase Ib/II trial of atezolizumab with cobimetinib or idasanutlin in metastatic estrogen receptor positive breast cancer.

NPJ breast cancer·2025

Same author

Highly multiplexed imaging reveals prognostic immune and stromal spatial biomarkers in breast cancer.

JCI insight·2025

Search research articles

Related Experiment Video

Updated: Jan 11, 2026

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

Contemporary Issues in Immunohistochemistry Utilization in Breast Pathology.

Melinda E Sanders¹

¹Department of Pathology, Vanderbilt Medical Laboratory of Vanderbilt University Medical Center, 445 Great Circle Road #1912, Nashville, TN 37228, USA.

Surgical Pathology Clinics

|November 12, 2025

Summary

Immunohistochemistry aids breast cancer diagnosis by identifying specific markers like GATA3 and TRPS1. Understanding staining patterns prevents misinterpreting conditions such as invasive lobular carcinoma and Paget disease.

Keywords:

E-cadherin GATA3 Lobular carcinoma Microinvasion Paget disease SOX10 TRPS1 Triple negative breast cancer

More Related Videos

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Related Experiment Videos

Last Updated: Jan 11, 2026

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Published on: January 21, 2019

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Published on: July 27, 2022

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Area of Science:

Oncology
Pathology
Biochemistry

Background:

Immunohistochemistry is crucial in diagnostic breast pathology.
Accurate interpretation of immunohistochemical markers prevents misdiagnosis of various breast conditions.

Purpose of the Study:

To highlight the utility of immunohistochemistry in breast cancer diagnosis.
To discuss specific markers for invasive lobular carcinoma, microinvasion, metastatic breast cancer, and triple-negative breast cancer.
To emphasize the importance of recognizing staining pattern variations in Paget disease.

Main Methods:

Review of current literature on immunohistochemical markers in breast pathology.
Analysis of marker expression patterns for specific breast cancer subtypes and conditions.

Main Results:

Aberrant E-cadherin expression is key for identifying invasive lobular carcinoma.
GATA3 is the most sensitive marker for metastatic breast cancer; TRPS1 shows slight superiority in primary breast tissue.
TRPS1 and SOX10 offer high specificity for triple-negative breast cancer.
Understanding variations in Paget disease staining patterns is vital for accurate diagnosis.

Conclusions:

Immunohistochemistry is indispensable for accurate breast cancer diagnosis.
Specific marker panels and knowledge of expression patterns improve diagnostic accuracy and prevent misinterpretation.
Context-focused staining and awareness of marker variations are essential for optimal patient care.