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Related Concept Videos

Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

863
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
863
Acute Coronary Syndrome III: Diagnostic Studies01:30

Acute Coronary Syndrome III: Diagnostic Studies

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Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
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Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

355
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
355
Coronary Artery Disease III: Clinical Manifestations01:30

Coronary Artery Disease III: Clinical Manifestations

313
Coronary Artery Disease (CAD) is a primary health risk worldwide, leading to significant morbidity and mortality. The condition arises from the buildup of atherosclerotic plaques within the coronary arteries, resulting in diminished blood supply to the heart muscle.The clinical manifestations of CAD vary widely, from asymptomatic stages to severe, life-threatening conditions. Understanding these manifestations is crucial for early diagnosis and effective management.Angina Pectoris: The Warning...
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Related Experiment Video

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Integrative Transcriptomic and Epigenomic Profiling for Signature Identification in Coronary Artery Disease: A Pilot

Mario Zanfardino1, Anna D'Agostino1, Ilaria Leone1

  • 1IRCCS SYNLAB SDN, 80143 Naples, Italy.

International Journal of Molecular Sciences
|November 13, 2025
PubMed
Summary

This study used multi-omics to find new molecular markers for coronary artery disease (CAD). Researchers identified a gene signature and regulatory patterns for better CAD risk stratification.

Keywords:
chromatin accessibilitycoronary artery diseaseepigeneticsmulti-omicstranscriptomics

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Area of Science:

  • Cardiovascular Research
  • Genomics and Epigenomics
  • Molecular Biology

Background:

  • Coronary Artery Disease (CAD) is a leading cause of death globally, driven by atherosclerotic plaques.
  • Despite advances, ~30% of initial CAD events remain fatal, highlighting the need for early detection.
  • Effective risk stratification is crucial for managing CAD patients.

Purpose of the Study:

  • To identify novel molecular markers for Coronary Artery Disease (CAD) using a multi-omics approach.
  • To uncover potential biomarkers for improved clinical risk stratification in CAD patients.
  • To investigate gene expression and chromatin accessibility patterns in CAD.

Main Methods:

  • Integrated transcriptomic (RNA-seq) and epigenomic (ATAC-seq) profiling of peripheral blood mononuclear cells (PBMCs).
  • Analysis of samples from individuals undergoing cardiac computed tomography angiography (CCTA).
  • Validation of key findings in an independent patient cohort.

Main Results:

  • Identified 39 genes consistently dysregulated across all CAD subtypes.
  • Revealed distinct chromatin accessibility patterns at CAD-associated loci.
  • Confirmed expression patterns of key Differentially Expressed Genes (DEGs), including Claudin 18 (CLDN18).

Conclusions:

  • Multi-omics data integration identified a core gene signature associated with CAD severity.
  • Distinct regulatory patterns were uncovered, offering potential biomarkers for clinical risk stratification.
  • Findings support the use of molecular markers for enhanced CAD management.