Cutaneous Wound Healing Facilitated by Postbiotic Extract Through Antimicrobial Action and Extracellular Matrix...

Area of Science:

• Microbiology
• Biotechnology
• Dermatology

Background:

• Wound healing is a complex biological process requiring tissue repair after injury.
• Postbiotics, derived from microbial fermentation, are emerging as potential therapeutic agents for wound management.
• Lactobacillus bulgaricus (L. bulgaricus) fermentation products offer promise in wound care applications.

Purpose of the Study:

• To evaluate the efficacy of a postbiotic extract from L. bulgaricus in promoting wound healing.
• To investigate the antimicrobial, immunomodulatory, and regenerative effects of the postbiotic extract.
• To assess the potential of postbiotics as a multifunctional approach to wound care.

Main Methods:

• Preparation of a postbiotic extract from L. bulgaricus via controlled fermentation, inactivation, and purification.
• In vitro assessment of antimicrobial activity against E. coli, S. aureus, and P. aeruginosa, and peptidomic analysis for antimicrobial peptides.
• Evaluation of effects on HaCaT cell proliferation, immune modulation, and collagen synthesis, alongside in vivo studies using a S. aureus-infected mouse wound model.

Main Results:

• The L. bulgaricus postbiotic extract demonstrated significant antimicrobial activity against tested pathogens.
• In vitro studies confirmed the extract's ability to enhance HaCaT cell proliferation, modulate immune responses, and stimulate collagen synthesis.
• In vivo experiments showed accelerated wound closure and increased collagen deposition in the treated mouse model.

Conclusions:

• The postbiotic extract from L. bulgaricus possesses significant antimicrobial, immunomodulatory, and regenerative properties.
• These findings support the use of composite postbiotics as a versatile and effective strategy for wound healing.
• Postbiotics represent a promising avenue for developing advanced wound care therapies.