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Hybrid Poly(Lactic)-Chitosan Scaffold Intensifying In Situ Bioprocessing of Rindera graeca Transgenic Roots for

Kamil Wierzchowski1, Szymon Bober1, Aleksandra Bandzerewicz2

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This study shows that hybrid poly(lactic)-chitosan scaffolds significantly boost rinderol production in Rindera graeca hairy roots. Fungal chitosan concentration is key for maximizing this bioactive compound

Keywords:
chitosan-based biomaterialhybrid biomaterialin situ bioprocessingnaphthoquinonestransgenic (hairy) roots

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Area of Science:

  • Plant biotechnology
  • Biomaterials science
  • Natural product chemistry

Background:

  • Rindera graeca, a rare endemic plant, produces bioactive naphthoquinones like rinderol.
  • Rinderol shows potential as an apoptosis inducer for cancer therapy.
  • Efficiently producing rinderol requires advanced bioengineering strategies.

Purpose of the Study:

  • To investigate the impact of hybrid poly(lactic)-chitosan scaffolds on Rindera graeca hairy root biomass and rinderol production.
  • To optimize scaffold composition, including chitosan origin, molecular mass, and concentration.
  • To compare scaffold performance against a reference culture system.

Main Methods:

  • Development of hybrid poly(lactic)-chitosan scaffolds with varying chitosan properties.
  • Cultivation of Rindera graeca hairy roots on these scaffolds.
  • Quantitative analysis of biomass proliferation and rinderol content.
  • Comparison with unmodified poly(lactic) acid scaffolds.

Main Results:

  • A PLA-chitosan scaffold with 33% fungal chitosan yielded a 635-fold increase in rinderol production (3660 µg gDW⁻¹).
  • Chitosan concentration within the hybrid scaffolds was identified as a critical factor for rinderol yield.
  • The developed scaffolds significantly enhanced rinderol production compared to reference systems.

Conclusions:

  • Hybrid PLA-chitosan scaffolds are effective for enhancing naphthoquinone production in Rindera graeca biomass.
  • Optimized scaffold design, particularly chitosan concentration, is crucial for maximizing bioactive compound yields.
  • This approach offers a promising strategy for renewable production of valuable plant-derived compounds.