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Updated: Jan 11, 2026

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Integrative Transcriptomic and Proteomic Analysis Reveals CaMK4-Mediated Regulation of Proliferation in Goat Skeletal

He Cong1, Lu Xu1, Yaolong Liu1

  • 1College of Animal Science and Technology, Southwest University, Chongqing 400715, China.

Animals : an Open Access Journal From MDPI
|November 13, 2025
PubMed

Insights

Calcium/calmodulin-dependent protein kinase 4 (CaMK4) regulates goat muscle stem cell proliferation and development. Understanding CaMK4’s network offers insights into improving goat meat production.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Ruminant Physiology

Background:

  • Calcium/calmodulin-dependent protein kinase 4 (CaMK4) is crucial for cellular signal transduction.
  • Its specific role in regulating goat skeletal muscle satellite cells (MuSCs) was previously unclear.

Purpose of the Study:

  • To elucidate the regulatory network of CaMK4 in goat MuSCs.
  • To investigate the impact of CaMK4 on muscle development and proliferation.

Main Methods:

  • Establishment of CaMK4 overexpression and knockdown models in goat MuSCs.
  • Integrated transcriptomic and proteomic analyses to identify regulatory pathways.
  • Bioinformatic analysis to pinpoint key regulatory nodes.

Main Results:

  • CaMK4 overexpression affected cAMP, PI3K-Akt, and actin cytoskeleton pathways; proteomic data indicated roles in calcium signaling and JAK-STAT pathways.
  • CaMK4 knockdown promoted MuSC proliferation by upregulating cell cycle genes.
  • Potential key regulatory nodes including LGALS9, CTHRC1, HAS1, and TDH were identified.

Conclusions:

  • CaMK4 plays a significant regulatory role in goat MuSC proliferation and development.
  • Findings provide a mechanistic framework for CaMK4 function in ruminant muscle.
  • This research may inform strategies to enhance goat muscle growth and meat quality.
Keywords:
CaMK4cell proliferationskeletal muscle satellite cells (MuSCs)transcriptomics and proteomics

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