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Updated: Jan 11, 2026
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
Published on: August 12, 2021
Indium Tin Oxide-Based Voltammetric Biosensor for the Detection of Antibodies Against the SARS-CoV-2 Virus Spike
Greta Zvirzdine1, Maryia Drobysh1, Almira Ramanaviciene1
1NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, 03225 Vilnius, Lithuania.
Abstract:
This study aims to propose a plausible application of a novel electrochemical biosensing system for detecting antibodies against SARS-CoV-2 (anti-rS) in serum samples. The uniqueness of this study lies in the biosensor utilizing recombinant spike glycoprotein (SCoV2-rS) immobilized on an indium tin oxide (ITO) electrode modified with (3-aminopropyl)triethoxysilane (APTES). The electrochemical performance was evaluated using square wave voltammetry (SWV), demonstrating a linear relationship between the current density and anti-rS concentration. The limit of detection (LOD) was found to be 113 ng/mL (0.75 nM), and the limit of quantitation (LOQ) was equal to 338 ng/mL (2.25 nM). The reported electrochemical biosensor offers a straightforward and efficient method for evaluating the immune status of individuals who have recovered from COVID-19 and been vaccinated against this virus without the need for any redox probe.
