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Published on: February 4, 2018
Rapid visual detection of tick borne Anaplasma marginale in Indian buffaloes using loop mediated isothermal
Niranjan Kumar1, Jayesh Babulal Solanki1, Dharmeshkumar Chandrakantbhai Patel1
1Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari, 396 450 India.
Abstract:
This study demonstrates the efficacy of loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays, both targeting the major surface protein 5 (msp5) gene, for the detection of Anaplasma marginale in Indian water buffaloes. The LAMP assay exhibited superior sensitivity, detecting the pathogen in 24 out of 110 samples (21.81%), compared to PCR, which identified 16 out of 110 samples (14.55%), and conventional microscopic examination, which detected only 6 out of 110 samples (5.45%). Relative to PCR, the LAMP assay achieved a diagnostic sensitivity of 93.75% (95% CI 69.77-99.84%) and specificity of 90.43% (95% CI 82.60-95.53%), with a positive predictive value of 62.50% and a negative predictive value of 98.84%. These findings highlight the LAMP assay as a sensitive, specific, and practical diagnostic tool for A. marginale in buffaloes, particularly suitable for field conditions due to its simplicity and visual detection capabilities.
