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CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects
Sarita Kumari1, Ankit Kumar Keshari1, Sumeet Kumar Singh2
1Department of Agricultural Biotechnology and Molecular Biology, CBS&H, RPCAU-Pusa, Samastipur, India.
Plant Molecular Biology
|November 13, 2025
Summary
Genome editing tools like CRISPR offer precise genetic improvements for legumes, enhancing their role in global food security. These advanced methods overcome traditional breeding limitations for faster, more effective crop enhancement.
Area of Science:
- Agricultural Science
- Plant Genetics
- Biotechnology
Background:
- Legumes are crucial global food crops, providing essential protein and contributing to food security.
- Traditional legume breeding methods are slow, labor-intensive, and limited by issues like linkage drag.
- Agroecological constraints and biotic/abiotic factors significantly impact legume production.
Purpose of the Study:
- To review modern genome editing tools for legume crop improvement.
- To assess the efficiency of various transformation methods in legumes.
- To discuss the current status, challenges, and future prospects of genome editing in legumes.
Main Methods:
- Overview of available genome editing technologies, focusing on CRISPR/Cas systems.
- Analysis of transformation efficiencies across different legume species.
- Review of recent advancements in genetic and genomic resources for legumes.
Main Results:
- CRISPR/Cas systems provide unprecedented precision and control for genome manipulation in legumes.
- Modern genome editing accelerates the improvement of legume crops compared to traditional breeding.
- Significant potential exists for targeted gene identification and manipulation to enhance legume traits.
Conclusions:
- Genome editing, particularly CRISPR, is revolutionizing legume genetic engineering and crop improvement.
- Addressing challenges in transformation and application is key to fully realizing genome editing's potential in legumes.
- Future research should focus on leveraging these tools for sustainable and resilient legume production.
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