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Related Concept Videos

Microbial Biosensors01:17

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
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Dual-Mode Biosensing Strategy Leveraging Single-Atom Fe Nanozyme for Cellular Glutathione Detection.

Fan Yang1,2, Chunyu Yan2, Deqing Chen2

  • 1Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan 030032, China.

ACS Applied Materials & Interfaces
|November 13, 2025
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Summary

A novel single-atom iron nanozyme (Fe SAzyme) enables dual-mode detection of intracellular glutathione (GSH). This sensitive and cost-effective biosensing strategy holds promise for point-of-care biomedical applications.

Keywords:
DNAzymeGSH detectiongene regulationredox dyshomeostasissingle-atom Fe nanozyme

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biochemistry

Background:

  • Intracellular glutathione (GSH) is crucial for understanding redox homeostasis and disease mechanisms.
  • Conventional GSH detection methods are often complex, costly, and lack sensitivity.
  • There is a need for efficient and accessible GSH detection strategies.

Purpose of the Study:

  • To develop a novel, dual-mode biosensing strategy for intracellular GSH detection.
  • To utilize a single-atom-dispersed Fe nanozyme (Fe SAzyme) with dual enzyme-mimetic activities.
  • To enable both UV-vis and smartphone-based RGB analysis for GSH quantification.

Main Methods:

  • A novel GSH sensing strategy employing Fe SAzyme with dual enzyme-mimetic activities.
  • Dual-mode detection using UV-vis spectroscopy and smartphone-based RGB analysis.
  • Application in distinguishing normal and cancer cells and observing GSH depletion in vitro and in vivo.

Main Results:

  • The Fe SAzyme-based strategy achieved dual-mode detection of GSH with distinct concentration ranges and limits of detection (LODs).
  • RGB mode: 150–1200 μM (LOD 110 μM); UV-vis mode: 10–150 μM (LOD 1.37 μM).
  • Successfully differentiated cancer cells from normal cells and demonstrated in vitro/in vivo GSH depletion, aligning with commercial kits.

Conclusions:

  • The Fe SAzyme-based dual-mode biosensing strategy offers a sensitive, cost-effective, and user-friendly approach for intracellular GSH detection.
  • This method shows significant potential for point-of-care biomedical diagnostics.
  • The study highlights the feasibility of inhibiting GSH regeneration for therapeutic interventions.