[The Applications of Hematoporphyrin in the Treatment of Multiple Myeloma]
Jin-Xing Wang1,2,3, Xiu-Juan Huang2, Qian Zou3
1School of Biomedical Engineering, Shenzhen University Medical School/NationalRegional Key Technology Engineering Laboratory for Medical Ultrasound/Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen 518060, Guangdong Province, China.
Objective:
Photodynamic therapy has become an important method in clinical tumor treatment. This study aimed to investigate the effects of hematoporphyrin on multiple myeloma (MM) and its potential applications.
Methods:
The MM cell line RPMI 8226 was treated with hematoporphyrin derivative (HPD), and CCK-8 assay was used to determine cell viability, apoptosis was detected by flow cytometry, intracellular reactive oxygen species (ROS) levels were measured using a detection kit combined with flow cytometry, and Western blot assay was used to detect apoptosis-related proteins and key signaling pathway protein levels.
Results:
The optimal incubation time for the maximum absorption of HPD in RPMI 8226 cells was 4 hours. HPD significantly inhibited the proliferation of RPMI 8226 cells in a dose- and illumination time-dependent manner ( r =0.981; r =0.961). Additionally, HPD induced apoptosis in RPMI 8226 cells, but had no significant inhibitory effect on peripheral blood mononuclear cells derived from healthy individuals. HPD combined with illumination treatment significantly increased the intracellular ROS level, upregulated the expression of apoptosis-related proteins such as cleaved PARP, cleaved caspase-3 and Bax, and down-regulated the expression of proteins that maintain cell survival, such as NF-κB and Akt.
Conclusion:
The HPD can inhibit the proliferation and induce apoptosis of multiple myeloma cells.
Insights
Hematoporphyrin derivative (HPD) effectively inhibits multiple myeloma (MM) cell proliferation and induces apoptosis. This photodynamic therapy shows potential for MM treatment by increasing reactive oxygen species and altering key protein expressions.
Area of Science:
- Oncology
- Photodynamic Therapy
- Molecular Biology
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