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    Area of Science:

    • Biomedical Optics
    • Optical Imaging
    • Microscopy

    Background:

    • High numerical aperture (NA) objective lenses in optical coherence microscopy (OCM) provide high lateral resolution but severely limit the depth of focus (DOF).
    • Computational methods to extend DOF in OCM often require high spatial-phase stability, which is challenging for point-scanning systems due to mechanical scanning-induced phase distortions.
    • Existing phase stabilization techniques are frequently complex and computationally demanding.

    Purpose of the Study:

    • To present a novel approach for overcoming the limited DOF in OCM.
    • To enhance the imaging capabilities of OCM by extending the effective DOF.
    • To provide a more practical solution for DOF extension in OCM systems.

    Main Methods:

    • Integration of self-reference interferometry to ensure inherent phase stability.
    • Application of a phase-sensitive computational refocusing algorithm.
    • Development of a combined approach leveraging the strengths of both techniques.

    Main Results:

    • Successfully addressed the limited DOF inherent in high-NA OCM.
    • Demonstrated effective DOF extension without compromising image quality.
    • The proposed method offers improved applicability compared to existing techniques.

    Conclusions:

    • The novel approach effectively extends the DOF in OCM by combining self-reference interferometry and computational refocusing.
    • This method offers a practical and computationally efficient solution for DOF extension in OCM.
    • The findings have significant implications for advanced optical coherence microscopy applications.