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R-loop accumulation promotes OSCC malignant progression by upregulating MARVELD3 expression.

Xinyi Li1, Xuerong Xiang1, Jiao Huang1

  • 1The Affiliated Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China; Chongqing Key Laboratory of Oral Diseases, Chongqing 401147, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.

Bioorganic Chemistry
|November 14, 2025
PubMed
Summary

R-loop accumulation in oral squamous cell carcinoma (OSCC) drives MARVELD3 expression, promoting tumor growth. MARVELD3 (Marvel domain-containing protein 3) activates the MAPK pathway by downregulating TTC9, highlighting a novel therapeutic target in OSCC.

Keywords:
MARVELD3Oral squamous cell carcinomaR-loopTTC9

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Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • R-loops are critical in cancer progression but their role in oral squamous cell carcinoma (OSCC) is unknown.
  • MARVELD3's function in OSCC, particularly concerning R-loops, requires investigation.

Purpose of the Study:

  • To investigate the role and mechanism of R-loop-mediated MARVELD3 in OSCC.
  • To explore how R-loops influence MARVELD3 expression and function in OSCC.

Main Methods:

  • DRIP-seq to detect R-loops in OSCC.
  • RT-PCR, IHC, and immunofluorescence to analyze MARVELD3 expression.
  • Functional assays (proliferation, migration, invasion) and molecular analyses (RNA-seq, Western blot, qPCR) to elucidate MARVELD3's mechanism.

Main Results:

  • R-loop accumulation correlates with MARVELD3 overexpression in OSCC.
  • MARVELD3 knockdown inhibits OSCC cell proliferation, migration, and invasion.
  • MARVELD3 promotes OSCC progression by suppressing TTC9 and activating the MAPK signaling pathway.

Conclusions:

  • R-loop accumulation upregulates MARVELD3 transcription in OSCC.
  • MARVELD3 promotes OSCC tumorigenesis via TTC9 downregulation and MAPK pathway activation.
  • Targeting the R-loop-MARVELD3 axis presents a potential therapeutic strategy for OSCC.