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Updated: May 4, 2026
Pre-clinical Model of Cardiac Donation after Circulatory Death
Published on: August 2, 2019
Current Methodological Procedures in Rat Models After Circulatory Death.
Mayara Munhoz de Assis Ramos1, Fernanda Yamamoto Ricardo-da-Silva2, Larissa Dos Santos Pedroso2
1Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), Instituto do Coração (InCor), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Ex vivo lung perfusion (EVLP) offers improved lung graft preservation and reduced ischemia-reperfusion injury (IRI) compared to traditional methods. Optimizing EVLP temperature, ventilation, and gas treatments enhances lung viability for transplantation.
Area of Science:
- Transplantation immunology and preservation science.
Background:
- Lung transplantation faces challenges with graft preservation and ischemia-reperfusion injury (IRI).
- Static cold storage is conventional but associated with complications.
- Ex vivo lung perfusion (EVLP) is an emerging alternative for graft assessment and treatment.
Purpose of the Study:
- To review advancements in EVLP for lung graft preservation.
- To explore strategies for minimizing IRI and improving graft viability.
- To discuss challenges and future directions for EVLP in lung transplantation.
Main Methods:
- Review of current literature on EVLP techniques and outcomes.
- Analysis of different EVLP temperature settings (hypothermic, subnormothermic, normothermic).
- Evaluation of ventilation strategies (negative-pressure vs. positive-pressure) and gas treatments (H2S, CO, NO) during EVLP.
Main Results:
- Normothermic EVLP shows potential in reducing IRI.
- Varied EVLP temperatures and negative-pressure ventilation may enhance lung preservation.
- Gas treatments during perfusion demonstrate promise for improved graft outcomes.
Conclusions:
- EVLP offers significant advantages over static cold storage for lung grafts, including evaluation and treatment capabilities.
- Further research is needed to understand graft injury mechanisms, refine EVLP protocols, and address cost-effectiveness.
- Development of animal models and alignment with clinical classifications are crucial for advancing EVLP application.
