Histological artifacts induced by the contrast agent in Multi-Phase Post-Mortem CT Angiography (MPMCTA): Part I -...

Area of Science:

• Forensic Pathology
• Medical Imaging
• Histopathology

Background:

• Multiphase postmortem computed tomographic angiography (MPMCTA) visualizes the cardiovascular system using contrast agents in cadavers.
• Understanding contrast-induced changes is crucial for accurate postmortem analysis.

Purpose of the Study:

• To systematically describe histo-morphological changes and artifacts from contrast agent mixtures (Angiofil® and paraffin oil) used in MPMCTA.
• To evaluate the impact of contrast media on histological findings.

Main Methods:

• Analysis of 278 histological samples from 37 cases undergoing MPMCTA and autopsy.
• Microscopic examination using an Olympus BX53 microscope and DP21 camera.
• Evaluation by three experienced forensic pathologists.

Main Results:

• Four main categories of artifacts were identified: optical empty spaces, compression of surrounding structures, hyperemia of small vessels, and vessel dilation.
• These artifacts are induced by the contrast agent injection process.

Conclusions:

• Postmortem angiography is valuable for vascular visualization.
• Contrast media can introduce significant histological artifacts.
• Careful consideration of these potential impacts is essential for interpreting MPMCTA results.