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Updated: Jul 30, 2026

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Published on: June 28, 2019
Protocol for culturing olfactory epithelium organoids supporting neuronal differentiation
Juliana Gutschow Gameiro1, Jussi Virtanen2, Liam Lee3
1Department of Clinical Surgery, State University of Londrina, Paraná, Londrina, Brazil; Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, and Graduate School of Biomedical Sciences, Boston, MA 02111, USA; Health Sciences Graduate Program, State University of Londrina, Paraná, Londrina, Brazil.
Abstract:
The olfactory epithelium (OE) contains stem cells capable of generating olfactory sensory neurons throughout life, making it a valuable model for studying epithelial neurogenesis. We present a protocol to develop a highly robust 3D mouse organoid model from OE cells. We describe a workflow for dissecting murine OE and subsequent organoid culturing. We also provide guidance on how to perform immunostaining of the organoids. This protocol has been validated for mice and does not require fluorescence-activated cell purification. For complete details on the use and execution of this protocol, please refer to Gameiro et al.1.
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