Related Experiment Video
Updated: Jan 11, 2026
Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay
Published on: November 10, 2013
Anticancer activity of the new manganese(II), iron(II), nickel(II), copper(II), and zinc(II) complexes based on
Agnieszka Czylkowska1, Suneel Lanka1, Bartłomiej Rogalewicz1
1Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Żeromskiego 114, Lodz 90-543, Poland.
Abstract:
In this study, we report the anticancer evaluation, synthesis, and characterization of a novel organic ligand, 4-butyl-5-((1-methyl-1H-pyrrol-2-yl)methyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione and its five metal complexes based on Mn(II), Fe(II), Ni(II), Cu(II), and Zn(II) ions. All compounds were analyzed using elemental analysis, NMR and FTIR spectroscopies, magnetic measurements, ESR, and thermogravimetric analysis. Their antioxidant properties were evaluated using the ABTS and DPPH assays, and the anticancer activity was studied in silico via molecular docking and in vitro against a panel of cancer cell lines. Notably, the Fe(II), Ni(II), and Cu(II) complexes exhibited potent anticancer activity, with IC50 values of 40.66, 45.95, and 26.51 µM against MDA-MB-231 cells. Their mechanism of action was further investigated through gene expression analysis, cell cycle analysis, apoptosis detection, thiol level evaluation, and DNA damage assessment. The results revealed that Ni(II) and Cu(II) complexes are significantly cytotoxic, while the Fe(II) complex acts as a potent cytostatic agent. Importantly, all compounds demonstrated lower activity toward normal cells than the tested cancer cells.
More Related Videos
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
07:20Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
Published on: May 28, 2014
Related Concept Videos
01:46Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
02:57Coordination Number and Geometry
02:51Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
03:02Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
01:29Chemotherapy-Induced Nausea and Vomiting: Dopamine Receptor Antagonists
Phenothiazines, such as prochlorperazine...
02:34Structural Isomerism
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...