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Updated: Jan 11, 2026
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Lipoxygenase inhibitory activity evaluation of Achillea biebersteinii Afan. by activity-guided fractionation
Bilgen Subaşi1, Tuğba Günbatan2, İlhan Gürbüz2
1Ministry of National Defence Pharmaceutical Factory Directorate, Ankara, 06110, Türkiye.
Ethnopharmacological Relevance:
Achillea biebersteinii Afan. is traditionally utilized as folk medicine for a broad range of therapeutic applications, especially for promoting the maturation of abscesses, wound healing; against inflammation, and rheumatism in Türkiye.
Aim Of The Study:
The anti-inflammatory potential of A. biebersteinii was evaluated through activity-guided fractionation (AGF) targeting lipoxygenase (15-LOX) inhibition.
Materials And Methods:
Different chromatographic techniques were used for the AGF of the ethyl acetate extract of A. biebersteinii aerial parts. The in vitro 15-LOX inhibitory activity evaluation was performed to address the anti-inflammatory activity. The structures of purified compounds from the fractions were confirmed by LC-HRMS, 1H NMR, and 13C NMR analyses, respectively.
Results:
The fractionation and isolation process culminated in the identification of three key flavonoids namely; patulitrin, axillarin, quercetagetin-7-O-β-glucopyranoside, and 4,5-dicaffeoylquinic acid, which showed statistically remarkable 15-LOX inhibitory activity with inhibition rates of 82.27 %, 96.81 %, 84.65 % and 77.47 %, respectively. Two flavonoids were isolated by using the AGF method, where quinic acid was spotted to have significant 15-LOX inhibitory activity.
Conclusion:
These findings support the future therapeutic potential of A. biebersteinii as a natural anti-inflammatory source.
