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Simplifying marine biotoxin data analysis: Advancing the Neuro-2a cell-based assay using R.

Synne T Frøstrup1, Christian Ritz2, Oliver Kappenstein3

  • 1German Federal Institute for Risk Assessment, Reference Center for Food and Feed Analysis, Unit Toxins, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany; Institute of Nutritional Science, Department of Food Chemistry, Arthur-Schenuert-Allee 114-116, 14558 Nuthetal, Germany.

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Summary
This summary is machine-generated.

A new R package, n2a, standardizes data analysis for the Neuro-2a (N2a) cell-based assay (CBA), improving marine biotoxin testing accuracy and efficiency for seafood safety.

Keywords:
BrevetoxinCiguatoxinDose-response analysisNeuro-2a bioassayR, data analysis

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Area of Science:

  • Environmental Science
  • Toxicology
  • Bioanalytical Chemistry

Background:

  • Marine biotoxins (MBs) are significant seafood contaminants posing public health risks.
  • The Neuro-2a (N2a) cell-based assay (CBA) offers a sensitive, high-throughput method for MB detection but lacks validation.
  • Data variability and processing errors challenge the N2a CBA's reliability.

Purpose of the Study:

  • To develop a standardized, validated data analysis procedure for the N2a CBA.
  • To address data variability and processing challenges in MB testing.
  • To enhance the accuracy and efficiency of seafood safety assessments.

Main Methods:

  • Development of a customized R package named 'n2a'.
  • Implementation of simultaneous dose-response analysis for N2a CBA data.
  • Automated calculation of effective concentration (EC50) and generation of graphical outputs.

Main Results:

  • The 'n2a' package provides the first standardized, free operating procedure for N2a CBA data analysis.
  • Rapid dose-response model fitting (2s per dataset vs. 3 min) significantly improves processing efficiency.
  • The package reduces potential data handling errors, enhancing data integrity.

Conclusions:

  • The 'n2a' R package is a crucial tool for validating and standardizing N2a CBA data analysis.
  • This advancement improves the reliability of MB testing in seafood, ensuring consumer safety.
  • The package facilitates cost-effective and efficient quality assurance for seafood products.