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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jan 6, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Urinary Micropeptides: Integrative In Silico Profiling and LC-MS/MS Analysis for Prostate Cancer Biomarker Discovery.

Rita Ferreira¹, Rui Vitorino^2,3

¹LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Aveiro, Portugal.

Methods in Molecular Biology (Clifton, N.J.)

|November 16, 2025

Summary

Micropeptides, small proteins from small open reading frames (sORFs), are emerging as key players in cellular functions. Research highlights their potential as noninvasive biomarkers for prostate cancer (PCa) detection and personalized medicine.

Keywords:

Micropeptides Noncoding RNA Proteomics Systems biology Therapeutic targets sORFs

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Last Updated: Jan 6, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

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Published on: August 2, 2018

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Area of Science:

Biochemistry and Molecular Biology
Cancer Research
Proteomics

Background:

Micropeptides, encoded by small open reading frames (sORFs), are a recently identified class of biomolecules with crucial cellular roles.
Historically overlooked due to technical and annotation challenges, advances in high-throughput techniques now facilitate their systematic study.
Prostate cancer (PCa) research is exploring urinary micropeptides as promising, noninvasive diagnostic and prognostic biomarkers.

Purpose of the Study:

To discuss methodologies for detecting and characterizing micropeptides.
To highlight recent discoveries of micropeptides relevant to prostate cancer.
To explore the potential of micropeptides in PCa diagnosis and personalized medicine.

Main Methods:

In silico prediction of micropeptides.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis.
Metabolic pathway enrichment analysis.

Main Results:

Identification and characterization of micropeptides using advanced proteomic techniques.
Discovery of differentially expressed micropeptides in prostate cancer.
Demonstration of micropeptides' potential as diagnostic and prognostic indicators for PCa.

Conclusions:

Micropeptide research is crucial for advancing our understanding of cellular functions.
Urinary micropeptides show significant promise as noninvasive biomarkers for prostate cancer.
These findings open new avenues for PCa diagnosis and the development of personalized treatment strategies.