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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jan 11, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers.

Tianqing Zhang¹, Xinmin Qian^2,3,4, Jiayi Zhang^5,6

¹Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, 310022, China.

Advanced Materials (Deerfield Beach, Fla.)

|November 17, 2025

Summary

This summary is machine-generated.

Researchers developed a rational design for synthetic allosteric DNA aptamers. This method precisely controls ON-OFF transitions, enabling tunable fluorescent properties for DNA computing and responsive nanostructures.

Keywords:

DNA computing DNA nanotechnology allosteric regulation fluorogenic DNA aptamer

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Area of Science:

Synthetic biology
Molecular engineering
Nanotechnology

Background:

Allosteric DNA aptamers offer precise molecular recognition.
Controlling their ON-OFF transitions is crucial for biosensing and computation.
Existing methods lack fine-tuned control over fluorescent properties.

Purpose of the Study:

To present a systematic approach for the rational design of synthetic allosteric DNA aptamers.
To achieve precise control over the allosteric ON-OFF transition in fluorescent DNA aptamers.
To engineer aptamers with highly tunable fluorescent properties.

Main Methods:

Rational design principles for synthetic allosteric DNA aptamers.
Integration with toehold-mediated strand displacement.
Development of aptamers where target sequences act as allosteric modulators.

Main Results:

Precise control over the allosteric ON-OFF transition was achieved.
Engineered aptamers exhibit highly tunable fluorescent properties.
Demonstrated applications in synthetic DNA computing and responsive nanostructures.

Conclusions:

The presented methodology enables rational design of allosteric DNA aptamers.
These aptamers can be precisely controlled for tunable fluorescence.
The aptamers are applicable in advanced DNA computing and responsive nanomaterials.